Coding

Part:BBa_K4940014

Designed by: Kuang Minjie   Group: iGEM23_iZJU-China   (2023-10-11)


P450BM-3 QM

P450BM-3 QM is a coding sequence of a type of Cytochrome P450 monooxygenases, which contains a quintuple mutant (V26T/R47F/A74G/F87V/L188K). In our projects, the enzyme can degrade α-pinene to α-pinene oxidation in a NADPH-dependent manner.

Usage and Biology

More specific information can be found in BBa K4940001 Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1373
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1373
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1373
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1373
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1373
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1219


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Categories
Parameters
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